Coding
Part:BBa_K4806015:Design
Designed by: Luca Langenberg Group: iGEM23_RPTU-Kaiserslautern (2023-09-21)
Hygromycin resistance for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 455
Illegal NgoMIV site found at 637 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimized for Chlamydomonas reinhardtii and compatible with Phytobricks/MoClo parts.
Source
Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PMID: 21364738; PMCID: PMC3041749.